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KMID : 0545120110210090954
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 9 p.954 ~ p.959
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Profiling of Recovery Efficiencies for Three Standard Protocols (FDA-BAM, ISO-11290, and Modified USDA) on Temperature-Injured Listeria monocytogenes
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Hai Yen Lee
Lay Ching Chai
Chai Fung Pui
Woan Chwen Wong
Shuhaimi Mustafa
Yoke Kqueen Cheah
Zuraini Mat Issa
Mitsuaki Nishibuchi
Son Radu
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Abstract
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There have been a number of studies conducted in order to compare the efficiencies of recovery rates, utilizing different protocols, for the isolation of L. monocytogenes. However, the severity of multiple cell injury has not been included in these studies. In the current study, L. monocytogenes ATCC 19112 was injured by exposure to extreme temperatures (60oC and -20oC) for a one-step injury, and for a two-step injury the cells were transferred directly from a heat treatment to frozen state to induce a severe cell injury (up to 100% injury). The injured cells were then subjected to the US Food and Drug Administration (FDA), the ISO-11290, and the modified United States Department of Agriculture (mUSDA) protocols, and plated on TSAyeast (0.6% yeast), PALCAM agar, and CHROMAgar Listeria for 24 h or 48 h. The evaluation of the total recovery of injured cells was also calculated based on the costs involved in the preparation of media for each protocol. Results indicate that the mUSDA method is best able to aid the recovery of heat-injured, freeze-injured, and heat-freeze-injured cells and was shown to be the most cost effective for heat-freeze-injured cells.
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KEYWORD
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Culturing method, Listeria monocytogenes, protocols, recovery, resuscitation, semilethal injury
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